Possibly from the Chapter House of S. Francesco, Siena, apparently from a large wall painting, perhaps of The Body of Saint Francis halted at S. Damiano, Assisi, and wept over by Saint Clare and her companions.

Ambrogio is recorded by Ghiberti and other writers as having painted frescoes in the cloister of the convent.

His brother, Pietro Lorenzetti, also contributed to the decorations.